Healthcare professionals have a key role to play in the introduction, application and use of technology in clinical practice. The lack of technical expertise and technological understanding poses a challenge to the quality of health services and probably to the safety, dignity and quality of life of patients. Experience so far speaks of computer literacy, which is the basis of the implementation of the health information system and varies according to gender, age and years of work experience. Determine whether computer literacy depends on gender, age, and years of service. The research was conducted according to the type of cross-sectional study, in the population of health workers employed at the Health Center in Jagodina and the General Hospital in Ćuprija. Yesterday’s sample consisted of 142 respondents. A structured questionnaire was used for data collection, statistical data processing was performed with the computer support of the statistical package for social sciences IBM SPSS Statistics, Version 23 (Statistical Package for Social Sciences). Analysis of variance was used from statistical tests. Results. Sample composed of 78.2% of respondents and 27.2% of respondents; in both groups of respondents, the average achievement on the literacy scale is around 17 points. Age 40 and over is 59%; the first two groups (less than 30 years of age and 30 to 39 years of age) are statistically significantly different from the oldest groups of respondents (F = 4.949, p = 0.003, df = 3). The largest percentage of respondents have a work experience of 10-29 years; younger respondents have more knowledge of computers (F = 7.239, p = 0.000, df = 3). Computer literacy depends on age and years of service.

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