In this study, we aimed to investigate the impact of radiation across a wide range of wavelengths, from UV-A to red visible light, on the role of neutrophils in inflammatory, autoimmune, and oncological diseases. Our focus was on understanding the photoacceptance process involving two cytochromes: cytochrome_b₅₅₈ and cytochrome_c oxidase. Through the utilization of Raman spectroscopy, we recorded characteristic Raman frequencies corresponding to various reactive oxygen species (ROS) and low-frequency lattice vibrational modes for citrulline. By employing selective inhibitors of NADPH oxidase (apocynin) and PAD4 (GSK484), we were able to establish that when neutrophils are exposed to light of different wavelengths, it activates signaling pathways that lead to the formation of NETs (neutrophil extracellular traps) through the involvement of NADPH oxidase and PAD4. During the irradiation of neutrophils, we observed distinct peaks indicating the presence of ROS and citrulline, suggesting the participation of intracellular ROS during light exposure. Development of novel drugs aimed at suppressing NETs formation could potentially inhibit NET formation at sites exposed to UV and visible light. This could result in a reduction in symptoms related to UV-induced photoaging and other forms of organ damage.

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