A study was carried out on the effect of 1 MeV accelerated electrons on the survival rate of suspensions of Escherichia coli bacteria and suspensions of Aspergillus fumigatus fungi at various initial concentrations and followed by plating on various nutrient media after irradiation. The samples were irradiated in the dose range from 0.15 kGy to 4 kGy. It was established that the concentrations of viable bacterial and fungal cells decreased nonlinearly with radiation dose. The doses required to reduce populations by a factor of 10 ranged from 0.20 kGy to 0.56 kGy for Escherichia coli at initial concentrations of 10³ CFU/g to 10⁵ CFU/g when plated on agar Thioglycollate medium; 1.28 kGy and 1.23 kGy for Aspergillus fumigatus at an initial concentration of 10⁶ CFU/g when plated on Sabouraud medium and Modified Czapek-Dox medium, respectively.

1. Estimating the burden of foodborne diseases, WHO, Geneva, Switzerland.
   Retrieved from: https://www.who.int/activities/estimating-the-burden-of-foodborne-diseases
   Retrieved on: Dec. 12, 2023
2. Food irradiation: Requirements for the development, validation and routine control of the process of irradiation using ionizing radiation for the treatment of food , ISO 14470:2011, Dec. 2011.
   Retrieved from: https://www.iso.org/standard/44074.html
   Retrieved on: Dec. 12, 2023
3. U. A. Bliznyuk et al., “Effect of electron and X-ray irradiation on microbiological and chemical parameters of chilled turkey,” Sci. Rep., vol. 12, no. 1, 750, Jan. 2022.
   DOI: 10.1038/s41598-021-04733-3
   PMid: 35031660
   PMCid: PMC8760279
4. U. A. Bliznyuk et al., “Determination of Chemical and Microbiological Characteristics of Meat Products Treated by Radiation,” Inorg. Mater., vol. 58, pp. 1422 - 1428, Dec. 2022.
   DOI: 10.1134/S0020168522140047
5. A. P. Chernyaev et al., “Study of the Effectiveness of Treating Trout with Electron Beam and X-Ray Radiation,” Bull. Russ. Acad. Sci.: Phys., vol.84, Moscow, Russia, Apr. 2020.
   DOI: 10.3103/S106287382004005X
6. A. Adu-Gyamfi, W. Torgby-Tetteh, V. Appiah, “Microbiological Quality of Chicken Sold in Accra and Determination of D10-Value of E. coli,” Food and Nutrition Sci., vol. 3, no. 5, pp. 693 – 698, May 2012.
   DOI: 10.4236/fns.2012.35094
7. C. H. Sommers, O. J. Scullen, S. Sheen, “Inactivation of uropathogenic Escherichia coli in ground chicken meat using high pressure processing and gamma radiation, and in purge and chicken meat surfaces by ultraviolet light,” Front. Microbiol., vol. 7, 413, Apr. 2016.
   DOI: 10.3389/fmicb.2016.00413
8. A. Xu, O. J. Scullen, S. Sheen, J. R. Johnson, C. H. Sommers, “Inactivation of extraintestinal pathogenic E. coli clinical and food isolates suspended in ground chicken meat by gamma radiation,”Food Microbiol., vol. 84, 103264, Dec. 2019.
   DOI: 10.1016/j.fm.2019.103264
   PMid: 31421757
9. J. Lee et al., “Radiation sensitivity of Aspergillus flavus in semi-dried beef jerky,” Food Sci. Biotech., vol. 13, no. 5, pp. 613 – 615, 2004.
10. N. H. Aziz, L. A. A. Moussa, F. M. E. Far, “Reduction of fungi and mycotoxins formationin seeds by gamma-irradiation,” J. Food Safety, vol. 24, no. 2, pp. 109 – 127, Jul. 2004.
    DOI: 10.1111/j.1745-4565.2004.tb00379.x
11. F. Hossain et al., “Radiosensitization of Aspergillus niger and Penicillium chrysogenum using basil essential oil and ionizing radiation for food decontamination,” Food Control, vol. 45, pp. 156 – 162, Nov. 2014.
    DOI: 10.1016/j.foodcont.2014.04.022
12. S. Aryal, “Microbial spoilage of meat and meat products,” Microbe Notes , Apr. 18, 2021.
    Retrieved from: https://microbenotes.com/microbial-spoilage-of-meat-and-meat-products/
    Retrieved on: Nov. 28, 2023
13. О. В. Фадейкина, “Аттестация стандартного образца мутности бактерийных взвесей,” Эталоны. Стандартные образцы, нo. 2, стр. 41 – 47, 2014.
    (O. V. Fadeikina, “Certification of a standard sample of turbidity of bacterial suspensions,” Certif. Reference Mater., no. 2, pp. 41 – 47, 2014.)
    Retrieved from: https://www.rmjournal.ru/jour/article/view/17
    Retrieved on: Nov. 28, 2023
14. U. Bliznyuk, N. Chulikova, V. Ipatova, A. Malyuga, “Effect of ionizing radiation with 1 MeV on phenology of potatoes inhabited by fungi Rhizoctonia solani Kuhn,” in Book of Abstr. Int. Conf. Advances in Agrobusiness and Biotechnology Research (ABR 2021), Krasnodar, Russia, 2021, 02001.
    DOI: 10.1051/e3sconf/202128502001
15. C. M. Ma, S. B. Jiang, “Monte Carlo modelling of electron beams from medical accelerators,” Phys. Med. Biol., vol. 44, no. 12, pp. 157 – 189, Dec. 1999.
    DOI: 10.1088/0031-9155/44/12/201
    PMid: 10616140
16. З. К. Никитина, И. К. Гордонова, Э. М. Насибов, “Изучение коллагенолитических свойств коллекционных штаммов микромицетов при длительном хранении,” Вопросы биол., мед. и фарм. химии, т. 24, нo. 3, стр. 33 – 39, 2021.
    (Z. K. Nikitina, I. K. Gordonova, E. M. Nasibov, “Study of the collagenolytic properties of collection strains of micromycetes during long-term storage,” Quest. biol. med. farm. chemistry, vol. 24, no. 3, pp. 33 – 39, 2021.)
    DOI: 10.29296/25877313-2021-03-05
17. “Микробиологическая чистота,” в XII Государственная фармакопея РФ, ч. 1, Москва, Россия: НЦЭСМП, 2007, сек. 32, стр. 160 – 193.
    (“Microbiological purity,” in XII State Pharmacopoeia of the Russian Federation , vol. 1, Moscow, Russia: Sci. Center for Expertise of Med. Products, 2007, sec. 32, pp. 160 – 193.)
    Retrieved from: https://docs.rucml.ru/feml/pharma/v14/vol1/
    Retrieved on: Nov. 28, 2023
18. R. R. O. Chirinos, D. M. Vizeu, M. T. Destro, B. D. G. M. Franco, M. Landgraf, “Inactivation of Escherichia coli O157:H7 in hamburgers by gamma irradiation,” Braz. J. Microbiol., vol. 33, no. 1, pp. 53 – 56, Jan. 2002.
    DOI: 10.1590/S1517-83822002000100011
19. B. M. Youssef, S. R. Mahrous, N. H. Aziz, “Effect of gamma irradiation on aflatoxin B1 production by Aspergillus flavus in ground beef stored at 5C,” J. Food Safety, vol. 19, no. 4, pp. 231 – 239, Dec. 1999.
    DOI: 10.1111/j.1745-4565.1999.tb00248.x
20. L. Fan et al., “Study on antibacterial mechanism of electron beam radiation on Aspergillus flavus,” Food Biosci., vol. 51, 102197, Feb. 2023.
    DOI: 10.1016/j.fbio.2022.102197
21. R. D. Jeong, E. J. Shin, E. H. Chu, H. J. Park, “Effects of Ionizing Radiation on Postharvest Fungal Pathogens,” Plant Pathol. J., vol. 31, no. 2, pp. 176 – 180, Jun. 2015.
    DOI: 10.5423/PPJ.NT.03.2015.0040
    PMid: 26060436
    PMCid: PMC4453998