Induced mutagenesis is one of the most used techniques to improve the quality of agricultural products. One of the main problems of induced mutagenesis is the assessment of treatment doses interval of biological materials for their processing. Calculation of radio-sensitivity is the first step in induced mutagenesis to improve the traits of different varieties. Our study is based on the selection of four varieties of winter bread wheat cultivated in Albania: DAJTI, UBT-1, UBT-2 and KIA-1. Irradiation of bread wheat seeds was carried out with X-rays generated by the RS-2400Q irradiation unit for the evaluation of radio-sensitivity tests. Referring to IAEA protocols, we chose for irradiation six X-rays doses of 50, 100, 150, 200, 300 and 400 Gy. The quantity of seeds for each treatment was the same and the biological material was planted in greenhouse pots. Seeds treated with 6 doses of radiation and the control were planted in three replicates each. To obtain an effective mutation with high probability, the growth reducing dose (GR50) and the lethal dose (LD50) were used in the radio-sensitivity test. The high radiation doses of 300 Gy and 400 Gy for X-rays caused significant damage to the seeds of the four bread wheat varieties. The KIA-1 variety was less resistant and at the 200 Gy, more than 75% of the plants were lost. Following the radio-sensitivity test, to study abiotic and biotic stresses in the experimental field, the three most appropriate doses to irradiate the seeds of these bread wheat varieties were 50, 100 and 150 Gy.

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