Vol. 4, 2019
Radiobiology
ANALYSIS OF THE CAPABILITIES OF THE PROGRAMS FIJI, IPLAB AND DARFI IN THE STUDY OF DNA REPAIR ABILITIES IN THE CELLS OF PATIENTS WITH A MOSAIC FORM OF ATAXIA TELANGIECTASIA
Aleksandra Nozdracheva, Roman Ushakov, Nadezhda Pleskach, Mirya Kuranova
Pages: 1-6
DOI: 10.37392/RapProc.2019.01
Abstract | References | Full Text (PDF)
Different programs are used for processing and analyzing of fluorescent images. However, some of these programs produce uncertain results due to their dependence on human factor. Here we made a comparative study of three programs used for fluorescent images analysis: IPLab, Fiji and DARFI. The programs were compared by the fluorescence intensity measurement quality and cell nucleus area detection. We also analyzed the quality of counting of foci in the cell nucleus, the determination of foci area and foci fluorescence intensity. Fluorescent images were obtained with laser microscope Zeiss Axiovert 200M after indirect immunofluorescence. The DNA repair study was performed on three cell lines from patients with ataxia-telangiectasia (AT). This disease is characterized by natural disruptions of the ATM kinase function. The study of the features of ATM kinase in DNA repair in patient cells is relevant not only in AT diagnostic, but also in the estimation of disease severity.
- E. Ledesma-Fernández, P. H. Thorpe, “Fluorescent foci quantitation for high-throughput analysis,” J. Biol. Methods, vol. 2, no. 2, Jul. 2015.
DOI: 10.14440/jbm.2015.62
PMid: 26290880
PMCid: PMC4538797 - J. M. Shillingford, IPLab Imaging Software for Microscopy from BD Biosciences version 4.0, Biocompare, South San Francisco (CA), USA, 2010.
Retrieved from: https://www.biocompare.com/Product-Reviews/41450-IPLab-Imaging-Software-For-Microscopy-from-BD-Biosciences/
Retrieved on: Aug. 22, 2019 - J. Schindelin et al., “Fiji: an open-source platform for biological-image analysis,” Nat. Methods, vol. 9, no. 7, pp. 676 - 682, Jun. 2012.
DOI: 10.1038/nmeth.2019
PMid: 22743772
PMCid: PMC3855844 - A. Sollazzo et al., “Live Dynamics of 53BP1 Foci Following Simultaneous Induction of Clustered and Dispersed DNA Damage in U2OS Cells,” Int. J. Mol. Sci., vol. 19, no. 2, Feb. 2018.
DOI: 10.3390/ijms19020519
PMid: 29419809
PMCid: PMC5855741 - E. Bobkova et al., “Recruitment of 53BP1 Proteins for DNA Repair and Persistence of Repair Clusters Differ for Cell Types as Detected by Single Molecule Localization Microscopy,” Int. J. Mol. Sci., vol. 19, no. 12, Nov. 2018
DOI: 10.3390/ijms19123713 PMid: 30469529
PMCid: PMC6321197 - T. Ferreira, W. Rasband, ImageJ User Guide – IJ 1.46r, National Institutes of Health, Bethesda (MD), USA, 2012.
Retrieved from: https://imagej.nih.gov/ij/docs/guide/user-guide.pdf
Retrieved on: Aug. 22, 2019 - M. L. Kuranova, N. M. Pleskach, T. A. Ledashcheva, V. M. Mikhel’son, I. M Spivak, “Mosaic forms of ataxia-telangiectasia,” Tsitologiia, vol. 56, no. 8, pp. 619 - 629, 2014.
PMid: 25697008 - И. В Озеров, “Математическое моделирование процессов индукции и репарации двунитевых разрывов ДНК в клетках млекопитающих при действии редкоионизирующего излучения с различной мощностью дозы,” к. ф.-м.н., МГУ имени М. В. Ломоносова, Радиобиология, Москва, Российской Федерации, 2015. (I.V. Ozerov, “Mathematical modeling of the processes of induction and repair of double-stranded DNA breaks in mammalian cells after the action of rarely ionizing radiation with different dose rates,” Ph.D dissertation, Lomonosov Moscow State University, Dept. of Radiobiology, Moscow, Russia, 2015.)
Retrieved from: http://www.bio.msu.ru/res/Dissertation/675/DOC_FILENAME/Ozerov_avtoref.pdf
Retrieved on: Aug. 22, 2019 - М. Г. Заднепрянец и др., “Влияние физических характеристик ускоренных тяжёлых ионов на формирование и репарацию двунитевых разрывов ДНК,” Письма в ЭЧАЯ., том. 15, но. 6(218), стр. 563 - 572, 2018. (M. G. Zadnipryanets, “The effect of the physical characteristics of accelerated heavy ions on the formation and repair of double-stranded DNA breaks,” Letters in JINR, vol. 15, no. 6(218), pp. 563 - 572, 2018.)
Retrieved from: http://www1.jinr.ru/Pepan_letters/panl_2018_6/17_zadnepryan.pdf
Retrieved on: Aug. 22, 2019