Several methods have been recommended in the literature for the radiochemical purity control of ^99mTc-mertiatide (MAG-3, mercaptoacetyltriglycine) radiopharmaceutical. This study aimed to compare these miniaturized chromatographic systems to verify their true usefulness and to determine the best way to quantify the radiochemical purity of this radiopharmaceutical, which is used in nuclear medicine for dynamic renal imaging for purposes of diagnosing the problems in kidneys. Instant Thin Layer Chromatography (ITLC) silica gel and Paper Chromatography using different solvents in different supports are analyzed. The chromatographic systems were: ITLC-SG, Whatman S&S 2589C, Whatman 4 (as support phase) in Acetone, NaCl, MEK, Absolute Alcohol, Ethyl Acetate:MEK 3:2, Acetonitrile 50% (as mobile phase). ITLC-SG was found to be the best support for these miniaturized chromatographic methods because it saves considerable time, fits quite well in the nuclear medicine department’s daily routine production, and meets the labelling criteria formulated by the manufacturer.

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   Retrieved on: Mar. 20, 2023